{"id":182,"date":"2015-03-12T17:35:43","date_gmt":"2015-03-12T17:35:43","guid":{"rendered":"http:\/\/reu.engr.uga.edu\/?p=182"},"modified":"2015-12-17T20:37:04","modified_gmt":"2015-12-17T20:37:04","slug":"gd-encapsulated-carbonaceous-nanodots-for-magnetic-resonance-imaging-mri-applications","status":"publish","type":"post","link":"https:\/\/reu.engr.uga.edu\/?p=182","title":{"rendered":"Design and Fabrication of a Microfluidic Device for the Live Imaging of Cells Encapsulated in Biomimetic Hydrogel Scaffolds"},"content":{"rendered":"<p><strong>Project Title: <\/strong>Design and Fabrication of a Microfluidic Device for the Live Imaging of Cells Encapsulated in Biomimetic Hydrogel Scaffolds<\/p>\n<p><strong>Project Duration:<\/strong> May 25 \u2013 August 1, 2015 (10 weeks), 40 hours per week.<\/p>\n<p><strong><span style=\"text-decoration: underline;\">Project Mentors <\/span><\/strong><strong> &#8211;<\/strong><\/p>\n<ul>\n<li><strong><span style=\"text-decoration: underline;\">Primary Faculty Mentor (Name, Affiliation, website and Email\/Phone): <\/span><\/strong><\/li>\n<\/ul>\n<p><strong>Lohitash Karumbaiah, Ph.D<\/strong><\/p>\n<p>Assistant Professor of Regenerative Medicine<\/p>\n<p>Regenerative Bioscience Center<\/p>\n<p>The University of Georgia<\/p>\n<p>ADS Complex | 425, River Road, Rm 310 | Athens, GA 30602-2771<\/p>\n<p>Ph: 706-542-2017| Fax: 706-583-0274 | Email: lohitash at uga dot edu<\/p>\n<p><a href=\"http:\/\/www.karumbaiahlab.org\/\">http:\/\/www.karumbaiahlab.org<\/a> |<a href=\"http:\/\/www.rbc.uga.edu\/\">http:\/\/www.rbc.uga.edu<\/a><\/p>\n<p>&nbsp;<\/p>\n<ul>\n<li><strong><span style=\"text-decoration: underline;\">Secondary Faculty Mentor (Name, Affiliation, website and Email\/Phone):<\/span><\/strong><\/li>\n<\/ul>\n<p><strong>Professor Leidong Mao<\/strong><\/p>\n<p>College of Engineering<\/p>\n<p>The University of Georgia, Athens<\/p>\n<p>Riverbend Research Laboratory South<\/p>\n<p>220 Riverbend Road Room 166<\/p>\n<p>Athens, GA 30602<\/p>\n<p>706.542.1871 \u2014 Tel (office)<\/p>\n<p>mao@uga.edu<\/p>\n<p>http:\/\/micronano.engr.uga.edu<\/p>\n<p>&nbsp;<\/p>\n<ul>\n<li><strong><span style=\"text-decoration: underline;\">Graduate Student\/PostDoc mentors (Name, Affiliation and Email\/Phone):<\/span><\/strong><\/li>\n<\/ul>\n<p><strong>Meghan Logun<\/strong><\/p>\n<p>Graduate Student<\/p>\n<p>Regenerative Bioscience Center<\/p>\n<p>The University of Georgia<\/p>\n<p>ADS Complex, 425, River Road, Athens, GA 30602-2771<\/p>\n<p>meglog@uga.edu<\/p>\n<p><strong><span style=\"text-decoration: underline;\">\u00a0<\/span><\/strong><\/p>\n<p><strong>Martha Betancur<\/strong><\/p>\n<p>Graduate Student<\/p>\n<p>Regenerative Bioscience Center<\/p>\n<p>The University of Georgia<\/p>\n<p>ADS Complex, 425, River Road, Athens, GA 30602-2771<\/p>\n<p>marthbet@uga.edu<\/p>\n<p><strong><span style=\"text-decoration: underline;\">\u00a0<\/span><\/strong><\/p>\n<p><strong><span style=\"text-decoration: underline;\">\u00a0<\/span><\/strong><\/p>\n<p><strong><span style=\"text-decoration: underline;\">Project Description:<\/span><\/strong><\/p>\n<p>While several studies have investigated tumor cell proliferation, and migration. Little is known about the tumor microenvironment and the influence, if any, of the biophysical interactions taking place in this milieu on cellular behavior. Hydrogels fabricated from natural or synthetic polymers can be used effectively to mimic the mechanical properties of tissue and the tumor microenvironment, and can potentially serve as ideal substrates to study tumor cell behavior in vitro. In this study, we hypothesize that glycosaminoglycan hydrogels can be modified to elicit a range of behaviors from encapsulated tumor cells, and that these outcomes can be visualized in real-time using a novel microfluidic device. This project is highly interdisciplinary in nature, involving microfluidics, biomaterials, Imaging, and cell and molecular biology. If successful, this platform technology can potentially be used to study stem cell behavior and can inform a host of other cell-transplantation therapies.<\/p>\n<p>&nbsp;<\/p>\n<p><strong><span style=\"text-decoration: underline;\">REU Student Role and Responsibility:<\/span><\/strong><\/p>\n<p>In this study, we hypothesize that glycosaminoglycan hydrogels can be modified to elicit a range of behaviors from encapsulated tumor cells, and that these outcomes can be visualized in real-time using a novel microfluidic device.<\/p>\n<p>&nbsp;<\/p>\n<p>The REU student will be responsible for:<\/p>\n<ol>\n<li>Working with a graduate student mentor in the Karumbaiah Lab, and personnel in Prof. Leidong Mao\u2019s lab to design the microfluidic imaging device.<\/li>\n<li>Cell culture of tumor cells and stem cells<\/li>\n<li>Hydrogel encapsulation of tumor cells and stem cells<\/li>\n<li>Labeling and live-cell imaging of encapsulated cells<\/li>\n<\/ol>\n<p><strong><span style=\"text-decoration: underline;\">\u00a0<\/span><\/strong><\/p>\n<p><strong><span style=\"text-decoration: underline;\">Expected Outcome for REU student:<\/span><\/strong><\/p>\n<p>If successful in our approach, the student\u2019s contribution on this project is expected to result in journal publications, conference presentations, and grant and patent applications in the near future.<\/p>\n<p><a href=\"https:\/\/reu.engr.uga.edu\/wp-content\/uploads\/2015\/03\/Tanasse-REU-Poster.pdf\">Tanasse REU Poster<\/a><\/p>\n<p><a href=\"https:\/\/reu.engr.uga.edu\/wp-content\/uploads\/2015\/07\/DSC_0146.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-490\" src=\"https:\/\/reu.engr.uga.edu\/wp-content\/uploads\/2015\/07\/DSC_0146.jpg\" alt=\"DSC_0146\" width=\"1280\" height=\"853\" srcset=\"https:\/\/reu.engr.uga.edu\/wp-content\/uploads\/2015\/07\/DSC_0146.jpg 1280w, https:\/\/reu.engr.uga.edu\/wp-content\/uploads\/2015\/07\/DSC_0146-300x200.jpg 300w, https:\/\/reu.engr.uga.edu\/wp-content\/uploads\/2015\/07\/DSC_0146-1024x682.jpg 1024w\" sizes=\"auto, (max-width: 1280px) 100vw, 1280px\" \/><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Project Title: Design and Fabrication of a Microfluidic Device for the Live Imaging of Cells Encapsulated in Biomimetic Hydrogel Scaffolds Project Duration: May 25 \u2013&#8230;<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[52,7],"tags":[31,28,4,41,59,5,60],"class_list":["post-182","post","type-post","status-publish","format-standard","hentry","category-2015-projects","category-research-projects","tag-brain","tag-cancer","tag-cell","tag-cell-communication","tag-microfabrication","tag-microfluidics","tag-nanotechnology"],"_links":{"self":[{"href":"https:\/\/reu.engr.uga.edu\/index.php?rest_route=\/wp\/v2\/posts\/182","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/reu.engr.uga.edu\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/reu.engr.uga.edu\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/reu.engr.uga.edu\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/reu.engr.uga.edu\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=182"}],"version-history":[{"count":4,"href":"https:\/\/reu.engr.uga.edu\/index.php?rest_route=\/wp\/v2\/posts\/182\/revisions"}],"predecessor-version":[{"id":642,"href":"https:\/\/reu.engr.uga.edu\/index.php?rest_route=\/wp\/v2\/posts\/182\/revisions\/642"}],"wp:attachment":[{"href":"https:\/\/reu.engr.uga.edu\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=182"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/reu.engr.uga.edu\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=182"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/reu.engr.uga.edu\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=182"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}