{"id":1102,"date":"2019-01-03T15:12:18","date_gmt":"2019-01-03T20:12:18","guid":{"rendered":"http:\/\/reu.engr.uga.edu\/?p=1102"},"modified":"2019-01-03T15:12:18","modified_gmt":"2019-01-03T20:12:18","slug":"investigating-neuronal-network-activity-in-functionalized-glycomaterial-scaffolds","status":"publish","type":"post","link":"https:\/\/reu.engr.uga.edu\/?p=1102","title":{"rendered":"Investigating Neuronal Network Activity in Functionalized Glycomaterial Scaffolds"},"content":{"rendered":"<p>Project Title: <strong>Investigating Neuronal Network Activity in Functionalized Glycomaterial Scaffolds <\/strong><\/p>\n<p>Project Mentors:<\/p>\n<ul>\n<li>Primary Faculty Mentor: Lohitash Karumbaiah<\/li>\n<\/ul>\n<p>Affiliation: University of Georgia, Regenerative Bioscience Center<\/p>\n<p>Ph: 706-542-2017<\/p>\n<p>Email: lohitash@uga.edu<\/p>\n<p>Website: <a href=\"http:\/\/www.karumbaiahlab.org\/\">http:\/\/www.karumbaiahlab.org<\/a>; <a href=\"http:\/\/www.rbc.uga.edu\/\">http:\/\/www.rbc.uga.edu<\/a><\/p>\n<p>&nbsp;<\/p>\n<ul>\n<li>Secondary Faculty Mentor: Leidong Mao<\/li>\n<\/ul>\n<p>Affiliation: University of Georgia, College of Engineering<\/p>\n<p>Ph: 706-542-1871<\/p>\n<p>Email: <u><a href=\"mailto:mao@uga.edu\">mao@uga.edu<\/a><\/u><\/p>\n<p>Website: <a href=\"http:\/\/magnet.engr.uga.edu\/\">http:\/\/magnet.engr.uga.edu<\/a><\/p>\n<p>&nbsp;<\/p>\n<ul>\n<li>Graduate Student mentor: Min Kyoung Sun<\/li>\n<\/ul>\n<p>Affiliation: University of Georgia, Regenerative Bioscience Center<\/p>\n<p>Ph: 412-980-7919<\/p>\n<p>Email: <u><a href=\"mailto:ms91136@uga.edu\">ms91136@uga.edu<\/a><\/u><\/p>\n<p>Project Description:<\/p>\n<p>Traumatic brain injuries (TBI) result in the acute release of the excitotoxic agent glutamate from damaged neurons, which causes neuronal network dysfunction and cell death. Studying neural network responses to excitotoxicity<em>in vivo <\/em>is challenging due to the complex TBI pathophysiological responses that complicate findings. We therefore propose to investigate the direct effects of excitotoxicity on neuronal activity <em>in vitro<\/em>using a microfluidic device platform and functionalized glycomaterials that mimic the structural and functional architecture of brain tissue. We will study neuronal network responses to excitotoxic challenge using calcium imaging and immunocytochemical assessments.<\/p>\n<p>REU Student Role and Responsibility:<\/p>\n<p>The student will perform glycopolymer functionalization and fabrication of hydrogels, fabricate microfluidic devices, maintain neural stem cell cultures, conduct immunocytochemical staining of cells, and perform live-cell calcium imaging of hydrogel encapsulated neurons. The student will also have the opportunity to process\/analyze neuronal spike profiles and images obtained using MATLAB.<\/p>\n<p>Required skills or courses:<\/p>\n<p>Prior experience in cell culture, a basic understanding of microscopy, any computer-aided design (AutoCAD, TinkerCAD) experience, and MATLAB experience.<\/p>\n<p>Expected Outcome for REU student:<\/p>\n<p>The student\u2019s contributions will be acknowledged in any resulting journal publications.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Project Title: Investigating Neuronal Network Activity in Functionalized Glycomaterial Scaffolds Project Mentors: Primary Faculty Mentor: Lohitash Karumbaiah Affiliation: University of Georgia, Regenerative Bioscience Center Ph:&#8230;<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[94,7],"tags":[],"class_list":["post-1102","post","type-post","status-publish","format-standard","hentry","category-2019-projects","category-research-projects"],"_links":{"self":[{"href":"https:\/\/reu.engr.uga.edu\/index.php?rest_route=\/wp\/v2\/posts\/1102","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/reu.engr.uga.edu\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/reu.engr.uga.edu\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/reu.engr.uga.edu\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/reu.engr.uga.edu\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1102"}],"version-history":[{"count":1,"href":"https:\/\/reu.engr.uga.edu\/index.php?rest_route=\/wp\/v2\/posts\/1102\/revisions"}],"predecessor-version":[{"id":1103,"href":"https:\/\/reu.engr.uga.edu\/index.php?rest_route=\/wp\/v2\/posts\/1102\/revisions\/1103"}],"wp:attachment":[{"href":"https:\/\/reu.engr.uga.edu\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1102"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/reu.engr.uga.edu\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1102"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/reu.engr.uga.edu\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1102"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}